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  • Initiation of bacteriophage T4 DNA replication and

    Dec 03, 2010 · Bacteriophage T4 initiates DNA replication from specialized structures that form in its genome. Immediately after infection, RNA-DNA hybrids (R-loops) occur on (at least some) replication origins, with the annealed RNA serving as a primer for leading-strand synthesis in one direction. As the infection progresses, replication initiation becomes dependent on recombination proteins in a process

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  • Publications - The Green Lab - UMBC

    Mutations of Bacteriophage T4 59 Helicase Loader Defective in Binding Fork DNA and in Interactions with T4 32 Single-stranded DNA-binding Protein*

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  • A Zinc Ribbon Protein in DNA Replication: Primer Synthesis

    Mutations of Bacteriophage T4 59 Helicase Loader Defective in Binding Fork DNA and in Interactions with T4 32 Single-stranded DNA-binding Protein. Journal of Biological Chemistry 2004, 279 (24), …

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  • A New Look at Old Mutants of T4 DNA Polymerase | Genetics

    Apr 01, 1998 · , 1994a Interaction of DNA polymerase and DNA helicase within the bacteriophage T4 DNA replication complex: Leading strand synthesis by the T4 DNA polymerase mutant A737V (tsL141) requires the T4 gene 59 helicase assembly protein. J. Biol. Chem. 268: 447 – 455.

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  • Interaction of the Bacteriophage T4 Gene 59 Helicase

    Sep 01, 2000 · Bacteriophage T4 gene 59 helicase loading protein accelerates the loading of T4 gene 41 DNA helicase and is required for recombination-dependent DNA r…

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  • Bacteriophage T4 gene 41 helicase and gene 59 helicase

    Jul 17, 2001 · T4 Gene 59 Helicase-Loading Protein. T4 mutants in gene 59 are UV-sensitive and defective in repair, recombination, and the recombination-initiated replication predominant in the late stage of infection (11, 12).The initial characterization of purified 59 protein showed that it was a small, monomeric, and basic protein that was capable of binding both single- and double-stranded DNA. 59

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  • Interaction of DNA polymerase and DNA helicase within …

    Background: Helicase loading protein Gp59 coordinates recombination-dependent DNA replication in bacteriophage T4.Results: A DNA binding-deficient mutant, Gp59-I87A, loads helicase normally onto ssDNA covered with the ssDNA-binding protein, Gp32.Conclusion: Gp59-Gp32 interactions control helicase loading onto D-loops during recombination-dependent replication.Significance: Helicase …

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  • Bacteriophage T4 Proteins Replicate Plasmids with a

    Mutations of Bacteriophage T4 59 Helicase Loader Defective in Binding Fork DNA and in Interactions with T4 32 Single-stranded DNA-binding Protein An amber mutation in gene 61, which encodes

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  • Bacterial and Phage Mutations That Reveal Helix-Unwinding

    Characterization of T4 Mutants Restricted on the optA Host Strain: DNA Helicase Mutants. The dda mutation L148 maps in an ill-characterized region of the T4 genome (8, 26). We tested other bacteriophage T4 strains, carrying mutations that map in the same region as dda, for restriction on the optA strain HR44.

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  • Exhibit Catalog - utoledo.edu

    Nov 30, 2020 · Mutations of Bacteriophage T4 59 Helicase Loader Defective in Binding Fork DNA and in Interations with T4 32 Single- Stranded DNA-binding Protein.? In The Journal of Biological Chemistry, 279 (24 Issue II), 25721-25728 (April 13, 2004).

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  • Mutations of bacteriophage T4 59 helicase loader …

    May 25, 2012 · Mutagenesis of T4 59 Helicase Loading Protein . Mutations in T4 gene 59 were made by site-directed mutagenesis of wild type (WT) gene 59 in the plasmid pNN2859, using the method previously described by Kunkel et al., modified by using T4 DNA polymerase, T4 gp44/gp62 clamp loader, and gp45 clamp to copy the ssDNA template. Primer sequences are available upon request.

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  • Control of Helicase Loading in the Coupled DNA …

    Jones, C.E., Green, E.M., Stephens, J.A., Muesser, T.C. and N.G. Nossal. Mutations of bacteriophage T4 59 helicase loader defective in binding fork DNA and in interactions with T4 32 single-stranded DNA-binding protein. J. Biol. Chem. 279: 25721-8, 2004.

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  • Templated Mutagenesis in Bacteriophage T4 Involving

    Mechanistic studies of the T4 DNA (gp41) replication helicase: functional interactions of the C-terminal tails of the helicase subunits with the T4 (gp59) helicase loader protein. J. Mol. Biol. 347 257–275. Drake, J. W., and L. S. Ripley, 1994. Mutagenesis, pp. 98–124 in Molecular Biology of Bacteriophage T4, edited by J. D. Karam. American

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  • HistCite: Bruce Alberts

    interaction of dna-polymerase and dna helicase within the bacteriophage-t4 dna-replication complex - leading-strand synthesis by the t4 dna-polymerase mutant a737v (tsl141) requires the t4 gene-59 helicase assembly protein. 177. 9575 1994 journal of biological chemistry 269(2):1284-1289 yonesaki t

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  • Involvement of a Replicative DNA Helicase of Bacteriophage

    Branch Migration at Holliday Junctions-The T4 59 helicase loading protein and the 41 helicase are both needed for recombination, as shown by the rec Ϫ phenotype of gene 59 mutants (2,42), and the

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  • Mutational Analysis of the T4 Gp59 Helicase Loader …

    Jun 03, 2005 · Bacteriophage T4 initiates origin-dependent replication via an R-loop mechanism in vivo.During in vitro reactions, the phage-encoded gp59 stimulates loading of the replicative helicase, gp41, onto branched intermediates, including origin R-loops. However, although gp59 is essential for recombination-dependent replication from D-loops, it does not appear to be required for origin …

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  • Break-Induced DNA Replication

    These PCNA mutations could be defective in the steps of establishing a full replication fork. Purification and characterization of bacteriophage T4 gene 59 protein: A DNA helicase assembly protein involved in DNA replication. Bacteriophage T4 helicase loader protein gp59 functions as gatekeeper in origin-dependent replication in vivo.

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  • Genes_Dev_2019_Oct_1_1329 | Bioz | Ratings For Life

    Searching further afield, bacteriophage T4 uses a helicase loader to initiate recombination-dependent DNA replication (RDR), the gp59 protein ( Kreuzer 2000). Remarkably, gp59 interacts with both the replicative helicase (gp41) and gp32, the ssDNA-binding protein analogous to RPA ( Ishmael et al. 2001 ); this tripartite interaction is key for

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  • Unraveling DNA helicases - Tuteja - 2004 - European

    Apr 27, 2004 · In the presence of DNA and ATP, the bacteriophage T4 gp41 hexamer binds six T4 gp59 molecules leading to a 1 : 1 helicase/helicase loader molar ratio []. Recent studies have shown that the hetero association interactions of T4 gp59 with ssDNA, T4 gp32 and T4 gp41 are all essential in the loading reaction [ [ 61 ] ].

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  • Role of gene 59 of bacteriophage T4 in repair of UV

    Nov 21, 2001 · Mutations of Bacteriophage T4 59 Helicase Loader Defective in Binding Fork DNA and in Interactions with T4 32 Single-stranded DNA-binding Protein. Journal of Biological Chemistry 2004, 279 (24), 25721-25728. DOI: 10.1074/jbc.M402128200. Charles E. Jones, Timothy C. …

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